Published January 5, 2017
Chronic Rodent Experiment
|March 17-19, 2017||Madrid, Spain||€1,500/person|
|NHP Surgical Technique with Matrix Array||May 2017||Montreal, Canada||Free to attend, USD $2,000 for hands-on surgery|
|NHP Electrophysiology using Vector Array||June 2017||Nashville, USA||Free|
Each workshop will be led by subject-matter research leaders and includes both in-depth discussion and some hands-on experience.
Workshops have limited spaces available and are subject to a minimum number of registered attendees.
Attendees are responsible for travel, room, and board during the workshop, in addition to the workshop cost.
NeuroNexus welcomes research laboratories willing to host a workshop. Contact us for more details, or if you have questions.
January 7, 2015
NeuroNexus recently collaborated with Shane Heiney of Javier Medina's lab at the University of Pennsylvania to design a custom component for their research with mice. Through the use of our in-house high-resolution 3D printing, we were able to arrive at a solution that improved the process and duration of Shane's experiments.
Tell us about your application and the problem your 3D print design was trying to solve.
"In our lab we make repeated daily acute microelectrode recordings from awake mice and needed a way to keep the craniotomy healthy between sessions. We had tried to chronically implant several different custom designed plastic rings to serve as 'recording chambers' but none of these designs offered a good way to completely enclose the craniotomy to keep air and infectious particles out. This forced us to perform dura peels almost daily to remove scar tissue and other 'gunk' that had accumulated between sessions.
What we needed was a chamber with a well-fitted locking cap."
Why did you choose 3D printing to produce your components?
"Recording chambers with locking caps are standard in non-human primate work and several vendors provide them, but we could find no vendors offering a similar design at a mouse scale. We thought this was a perfect use case for 3D printing."
How was your experience working with NeuroNexus?
"We designed a custom recording chamber with interlocking lid and approached NeuroNexus to see if they would be able to print it for us. Our primary concern was that some of the features would be too small even for their high resolution printer to handle. Their engineers worked with us through several iterations of the design to come up with a finished product that is far more robust than our original design. They even suggested a new feature that turned out to be critical for holding the cap in place while the mouse is in its home cage between experiments."
Tell us about the results of the collaboration.
"We have been very pleased with the results of this collaboration and the newly designed chambers have greatly increased the health of the dura between sessions. We've found that by adding a small amount of silicone elastomer to the dura between experiments, which the cap prevents from falling out, we have eliminated the need for daily dura peels. Our implants routinely last for several months."
Published June 1, 2013
The Uniklinik Freiburg hosted a two-day neuronal recording workshop in May, and Senior Applications Engineer Andre Snellings was our man on the (German) ground.
The workshop was targeted at researchers taking their first steps into neuronal recording. Working with five different recording setups, researchers progressed beyond single electrode work to inserting and recording with multi-channel electrodes.
The workshop had two major components each day: a morning lecture where participants were taught about neural electrophysiology in presentations, and an experimental component that made up the remainder of the day. The 20 participants were broken into five groups of four students each, which would comprise their lab group for the duration of the workshop.
The experimental portion of the workshop happened at five different work stations, spread over two rooms. Each work station was led by a representative from one of five data acquisition companies (Tucker Davis Technologies, Plexon Inc., Black Rock Microsystems, Alpha Omega, and Multichannel systems) as well as two research assistants. The research assistants at each station performed surgery on a rat model, inserting a multisite recording electrode into a unique spot in the brain. Once the probe was placed, the data acquisition rep used their recording system to identify potential neural signals. At the end of each two hour session, the rep and RAs would stay in place and the lab group would move to another station.
The sensory area of the thalamus was a popular recording location, because putative cells from that area could fire in response to an experimenter touching the rat’s whiskers. In the picture on the left, the blue neuron identified on the monitor would fire faster in response to whisker palpation and slower else.
One group recorded from the hippocampus. The hippocampus is a popular recording location in awake, behaving animals because it is a central location for learning and memory. However, in the workshop environment with anesthetized rats, the hippocampus doesn’t allow the same type of easy stimulation/response as shown with the whisker response above. Nevertheless, Victor from Tucker Davis and the two research assistants (all shown in the photo on the right) were able to identify a few neurons.
Close-up detail, Insertion methods, and more
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