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Custom Design

Dr. Antonio Paolini
La Trobe University, Australia

Paolini

The 3-D probe enables unparalleled ability to map neural responses across an entire structure.

~Dr. Antonio Paolini

Dr. Paolini’s focus is currently directed toward understanding how auditory and olfactory information is processed through the amygdala. The presentation of olfactory stimuli can be laborious, and since cells adapt relatively quickly to the stimuli generating enough trials to ascertain the neural profile to a smell can be difficult. The 3-D probe allows Dr. Paolini to simultaneously map a large portion of the structure of interest and obtain a profile of the neural responses across 128 channels. The custom NeuroNexus 3D structure allowed the electrode shanks and sites to be configured to maximize sampling efficiency. A strong feature of the array is that it robust enough to allow deep brain recording from predictable trajectories.

ResultS

One of the most significant advantages of this custom design is that it allows electrodes to be inserted deep within the brain with little deviation from the 3-D configuration of the array. The figure below shows the ease of insertion of the 16 electrode prongs arranged in a 4 X 4 grid (A-C). The electrodes have been dipped in DiI allowing histological verification of recording positions (D-F). Electrodes remained in their 4x4 configuration deep within the brain indicated by dots (showing DiI locations) superimposed onto Fluorescent Nissl stained sections (E,F). The figure at right shows the response of 16 of the 128 sites responding to an odor stimulus.

Probe Status

3D arrays are customizable by choosing any set of 2D arrays to fit your experimental needs.

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