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Custom Design

Dr. Yoshikazu Isomura
Tamagawa University, Japan

Isomura

I feel truly grateful for being introduced to NeuroNexus probes and multi-neuron recording techniques in Dr. Gyorgy Buzsaki’s lab. The probe I designed allows simultaneous comparison of cell-firing activity between different layers of cortex from rats or mice, in one experimental session. Moreover, it’s suitable for CSD analysis of LFPs. I would recommend this for your first recording from cortex.

~Dr. Yoshikazu Isomura

Dr. Isomura investigates the microcircuitry and mechanisms in behaving rat primary motor cortex underlying preparation, initiation, and expression of voluntary movements. His 16-channel custom probe permits him to simultaneously record local field potentials (LFPs) and spike activity from multiple single neurons across cortical layers. His probe includes nine recording sites for LFPs (0 to 1,200 µm deep at 150 µm intervals) and two tetrode configurations (400 and 1,200 µm deep) for recording spike activity at superficial and deep layers. The two tetrode configurations are placed on leading edges of the array for interfacing with healthy tissue. This probe provides valuable information on functional processing in superficial and deep layers through simultaneous single-cell and population recordings.

Results

Dr. Isomura and his colleagues performed multi-neuron and LFP recordings from various layers of the motor cortex during forelimb movements. They showed different motor-related functions of pyramidal cells and interneurons across the cortical layers (Nat. Neurosci., 12: 1586-1593, 2009). More recently, they have found that spiking activity of motor cortex neurons was often phase-locked to slow and fast gamma oscillations in the LFP activity, which might be functionally associated with motor preparation and motor expression, respectively.

Probe Status

The electrode design is available in the catalog as a special order. (Contact us for more detail.) Dr. Isomura appreciates maintaining contact with users of his custom design and learning about other applications and results.

LFP8-Tetrode SD probe:

probe

signal-graph

probe-in-use