Drs. Hong Lei and Cécile FaucherWashington University, USA
Having such a high number of recording sites in 2 locations will significantly increase the chance to detect synchronous activity in one region of the moth brain and observe correlated responses in the target region.~Drs. Hong Lei & Cécile Faucher
The University of Arizona group is interested in how olfactory information is processed in insect brains, more particularly how synchronous activity in the antennal lobes is detected by downstream protocerebral neurons. They therefore needed to record simultaneously from two different locations in the small moth brain. NeuroNexus was tasked to design a high density probe to allow access to such a small brain region without excessive damage. A 64-channel array was designed that was comprised of a 3D stack of three 16-channel A-Probes and a single 16-channel A-Probe with flexible cable attached. The two units were integrated to one single connector, allowing access to two brain regions while minimizing the bulk of the probe assembly.
Antennal lobe output neurons converge onto lateral protocerebrum, resulting in less specific responses at this synaptic level. The correlation between such converging inputs and responsiveness of protocerebral neurons is currently being characterized.
3D arrays are customizable by choosing any set of 2D arrays to fit your experimental needs.
Schematic Representation of a NeuroNexus 3D Probe in the Moth Brain
Simultaneous Recordings from the Antennal Lobes (upper 5 traces) and Protocerebrum (lower trace)
A session of recordings from the 3D Probe Assembly