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Science Update

Recording extracellular neural activity in the behaving monkey using a semichronic and high-density electrode system

Publication Details

Featured Authors

German Mendoza

Hugo Merchant

All Authors
Adrien Peyrache, Jorge Gamez, Luis Prdo, Gyorgy Buzsaki
Journal Name
J Neurophysiology
Other Publisher Name
Innovative Methodology
Experiment Type
Chronic Microdrive
Animal Model
Monkey
Brain Region
Cortex Motor
Brain Region
pre-SMA
Brain Signal
Single Unit
Product Category
  • Penetrating Electrode
  • Primate Electrode
Product
  • Buzsaki64
Probe Detail/Package
Z64
Abstract
We describe a technique to semichronically record the cortical extracellular neural activity in the behaving monkey employing commercial high-density electrodes. After the design and construction of low cost microdrives that allow varying the depth of the recording locations after the implantation surgery, we recorded the extracellular unit activity from pools of neurons at different depths in the presupplementary motor cortex (pre-SMA) of a rhesus monkey trained in a tapping task. The collected data were processed to classify cells as putative pyramidal cells or interneurons on the basis of their waveform features. We also demonstrate that short time cross-correlogram occasionally yields unit pairs with high short latency (<5 ms), narrow bin (<3 ms) peaks, indicative of monosynaptic spike transmission from pre- to postsynaptic neurons. These methods have been verified extensively in rodents. Finally, we observed that the pattern of population activity was repetitive over distinct trials of the tapping task. These results show that the semichronic technique is a viable option for the large-scale parallel recording of local circuit activity at different depths in the cortex of the macaque monkey and other large species.
Citation
G. Mendoza et al. "Recording extracellular neural activity in the behaving monkey using a semichronic and high-density electrode system." J Neurophysiol. 2016 Aug 1;116(2):563-74. doi: 10.1152/jn.00116.2016
Publication Date
08/ 1/2016
Country
United States
Institution
University of Mexico