Organoid Electrophysiology
Penetrating 3-D recording inside brain organoids, with Radiens spike sorting, burst metrics, spectral power and cross-organoid synchrony. The initial system is launching now. A higher-throughput system is in development.
Find your way around
A brain organoid is a three-dimensional neural tissue grown from human stem cells. A planar array records from the surface it rests on; the neurons that fire are distributed through the volume. The platform places electrode sites inside the organoid, so the recording samples the tissue, not its surface.
The platform
An electrode array built for the organoid, the interface that reads it, and the software that records and analyzes it. Engineered together, from the company that has made thin-film neural probes for 22 years.
01.
Electrode array
Thin-film silicon sites positioned to penetrate the organoid, made by the same microfabrication process as the in-vivo catalog.
How a probe is made02.
Interface and acquisition
The array terminates in a connector a headstage mates; the headstage digitizes every site and the data acquisition system streams the recording to the host.
The recording chain03.
Software
Radiens Allego records and monitors signal quality live; Radiens Videre sorts spikes and computes burst, spectral and synchrony metrics after the recording.
RadiensWhat it measures
Readouts a lab reports today, computed in Radiens from the same recording.
Sorted units
Each recorded spike assigned to the neuron that produced it, so activity is counted per unit rather than per channel.
Burst metrics
Burst rate, duration and spikes per burst from each unit’s spike train, the measures organoid maturation is tracked by.
Spectral power
Power by frequency band and time on every site, for the network oscillations an organoid develops.
Cross-organoid synchrony
How the activity of two organoids, or two regions of one, lines up in time.
Signal quality, live
Noise and signal-to-noise on every site during the recording, so a failing site is found during the session.
Provenance on every file
A unique id and a provenance list on every recording, so any result is re-derivable.
Who it is for
You grow organoids, slices or cultures and need activity from inside the tissue: development, network formation, disease models and compound response, recorded over weeks.
Request early accessYour readouts inform decisions, so they have to be reproducible and defensible: sorted units, burst and spectral metrics with provenance on every file. A higher-throughput system is in development.
Talk to a scientistThe research behind it
NeuroNexus runs NIH-funded research programs in-house and uses its own devices in them. The organoid electrophysiology program is run with Harvard. The platform is what that program produced.
Radiens for organoids
Organoid recordings open in the same apps as in-vivo work. Radiens reads multi-electrode-array data through its model builder, Allego records and monitors signal quality live, and Videre runs spike sorting, PSTH, spectral and field analysis with provenance.Radiens Standard is free to start. Data stays in the lab’s own storage.
Target-centered views
Site-by-site activity drawn on the array’s geometry, for a culture or a slice as for a brain.
Reads your existing files
Intan, SpikeGLX, Open Ephys, TDT, Plexon and NWB, natively; NWB written as well as read.
Scriptable
RadiensPy and the Radiens CLI drive acquisition and analysis from Python and Jupyter.
In development: a higher-throughput successor to the initial system, for screening at scale. It is direction, not a shipping product; ask us about timing when you request early access.
Organoid Electrophysiology — FAQ
Can I record from brain organoids with NeuroNexus hardware today?
The initial NeuroNexus organoid electrophysiology system is launching now, and a higher-throughput system is in development; contact NeuroNexus for availability. Radiens supports multi-electrode array data from organoids, cultures and slices through its model builder today.
How is organoid recording different from in-vivo probes?
3-D tissue needs arrays that interface across the organoid volume, with chronic protocols for maturation tracking rather than acute insertion.
What software analyzes organoid electrophysiology data?
Radiens handles spike sorting, burst metrics, spectral power, and cross-organoid synchrony from the same acquisition runtime.