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Two mouse EEG thin-film grids: polyimide arrays with platinum disc sites and a crosshair fiducial for alignment to bregma

Mouse EEG Electrodes and Grids

Thin-film EEG grids for the mouse skull: 16, 30 or 32 platinum sites aligned to bregma, placed with no craniotomy.

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Cited in published research

The mouse EEG grids

Select a grid by site count and cable length; each opens its datasheet.
GridSitesSite outlineCablePackagesAvailability
EEG 30-Channel GridEEG-M-30-A-CL10306.9 × 8.8 mm10 mmH32 + 5 more
EEG 30-Channel GridEEG-M-30-A-CL7306.9 × 8.8 mm7 mmH32 + 5 more
EEG 30-Channel GridEEG-M-30-A-CL8306.9 × 8.8 mm8 mmH32 + 5 more
EEG 32-Channel GridEEG-M-32-B-CL10-RET328.0 × 6.5 mm10 mmH32 + 5 more
EEG 16-Channel GridEEG-M-16-CL10167.5 × 8.1 mm10 mmH16 + 5 more

The grid

A 12 µm polyimide film carries 500 µm platinum sites drawn to the mouse skull, so each site lands on the same region in every animal.

No craniotomy

The film sits on the intact skull; bone screws clear of the grid carry the load and give ground and reference.

Aligned to bregma

A crosshair on the film aligns to bregma, so placement is one step and repeats from animal to animal.

Weeks to months

The implant holds for weeks to months, so spectral and coherence measures follow the same animal.

Freely moving

The grid ends in an H32 or H16 Hybrid package and a headstage, for recording awake, freely moving mice.

The 30-site mouse EEG grid layout, drawn to the mouse skull and referenced to bregma

What it connects to

Each grid mates directly with a NeuroNexus headstage and records on any NeuroNexus acquisition system, with no adapter.

01.

Package: H32 or H16

The 30- and 32-site grids end in an H32 Hybrid package, the 16-site grid in an H16; both are Omnetics.

02.

Headstage: SmartLink or X3R

An H32 mates SmartLink 32 or X3R 32; an H16 mates SmartLink 16.

03.

Commutator, for long sessions

A Standard Commutator or Commutator PRO keeps the tether from twisting as the mouse moves.

04.

Acquisition: SmartBox Pro or XDAQ

Each of these headstages runs on SmartBox Pro and on XDAQ ONE+, CORE2 and ALL-IN-ONE.

05.

Software: Allego and Radiens

Allego acquires and Radiens analyzes on every one of these systems.

How to choose

The choice turns on how many places on the skull the question needs, and how alike they must be between animals.

Bone-screw electrodes

One channel per screw, placed by hand: the simplest route to a seizure count or a sleep score.

Thin-film grid

16 to 32 sites placed in one step on the same region in every animal, for spectral and coherence phenotypes.

Wireless headmount

No tether, but the transmitter sets the channel count, usually a few channels on screw electrodes.

Published with these grids

Mouse studies on the 30-site grid, and the rat layout of the same series.

Jonak et al., 2018

Reusable multielectrode array technique for EEG in awake, freely moving mice. Front Integr Neurosci.

Read more research

Mouse EEG — FAQ

No. The thin-film grid sits on the intact skull over the recording area, with no bone removed. Bone screws placed clear of the grid anchor the implant and provide the ground and reference, and the grid is cemented at its edges and at the cable exit.

It records for weeks to months, so a biomarker can be followed through development, or a treatment measured against a baseline, on the same implant. Impedance that rises over weeks usually means the cement is lifting at an edge.

The 30- and 32-site grids end in an H32 Hybrid package that mates a 32-channel headstage such as SmartLink 32 or X3R 32; the 16-site grid ends in an H16 for SmartLink 16. Each runs on Smartbox Pro or XDAQ, with Allego for acquisition and Radiens for analysis.

Yes. Jonak et al. (2018) describe a reusable 30-channel technique for EEG in awake, freely moving mice on the NeuroNexus mouse grid. Handling and cleaning between uses are in Thin-Film Grid Electrodes: Setup, Handling, Cleaning and Care.

Matching a grid to your rig

An Application Scientist can match the package and headstage to your acquisition system, and replies within one business day.

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